Our lab uses the TeT-on system to exert spatial and temporal regulation on the Hedgehog signaling system. We have been having some penetration issues with doxycycline in older fish, so we decided to inject doxycycline into the ventricles to see if we could activate the Tet system. As a test-run, we decided to practice some injections with a fluorescent bead, called Dextran. After several tests with injection needles, dosage and injection sites, we got Dextran into one fish! Hopeful?! This is just step-one of several trial steps to go, but it seems like we are heading in the right direction.
This is a ventral view of dissected zebrafish brain at 4 days post fertilization. EdU treatment followed by a “click” chemical reaction enables us to visualize cells (the green dots) that are dividing in the zebrafish brain.